References
Related tools:
PRIDE
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Primer Design for DNA-arrays/chips (GenomePRIDE 1.0)
GenomePRIDE is primer design program that designs PCR primers or long oligos on an annotated sequence.
So far, GenomePRIDE was used for the design of DNA arrays/chips containing all ORFs of the following organisms:
Bacillus subtilis (4100, full ORF primer pairs, >>97% success)
Schizosaccharomyces pombe (~5000, ORF-specific primer pairs, >99% success)
Drosophila melanogaster (Heidelberg FlyArray, 21000, ORF-specific primer pairs, >99% success)
Candida albicans
etc.
The program is tightly integrated into the Staden package (GAP4).
FEATURES (preliminary online manual)
high record of success
computation of PCR primers and/or oligos
fast computation
defined fragment length
homologies are analyzed
visualization (based on the Staden package)
design of primers in a specific frame
Availability
Stefan Haas
Last modified: Jun 2006
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